Tag Archives: Environmental Monitoring

Tech Tip: Hydrating 3M™ Petrifilm™ Plates

 

Source: 3M

Source: 3M

When preparing 3M™ Petrifilm™ for use in your environmental monitoring program there are different hydration methods to follow, depending on the test and the context in which it is used.

For air or direct contact procedures where Aerobic Count, Coliform Count, E.coli/Coliform Count, Rapid Coliform Count or Enterobacteriaceae Count Petrifilm™ are being used, hydrate plates with 1 mL of appropriate sterile diluent. Allow hydrated plates to remain closed for a minimum of 1 hour before use.  If using Staph Express Count Petrifilm™, hydrate plates with 1 mL of appropriate sterile diluent. Refrigerate hydrated plates for a minimum of 3 days before using.

For air testing using either Yeast and Mold Count or Rapid S. aureus Count Petrifilm™, hydrate plates with 1 mL of appropriate sterile diluent. Allow hydrated plates to remain closed for a minimum of 1 hour before use.

For direct contact methods only using Yeast and Mold Count Petrifilm™, hydrate plates with 1 mL of sterile letheen broth only. Place letheen inoculated plates into sealed bag and incubate at 30-37°C (86-99°F) for 24 hours. After incubation, store sealed bag of plates in refrigerator for a minimum of 4 hours to allow gel to solidify. Plates hydrated with letheen will have a mottled appearance.

If you prefer using the new Rapid Aerobic Count Petrifilm™ the hydration times are slightly longer for air or contact surface monitoring. Air sampling has a 24-hour minimum hydration period refrigerated. After hydration plates can be stored up to 7 days. Contact sampling has a 3-day minimum hydration period refrigerated. These plates may also be stored for 7 days after hydration.

Refer to relevant package insert for details and listing of appropriate diluents.

For storage purposes, place all hydrated Petrifilm™ in sealed pouch or plastic bag. Protect plates from light and refrigerate at 2-8°C (36-46°F). Hydrated Petrifilm™ Aerobic Count plates may be refrigerated up to 14 days, all other hydrated Petrifilm™ plates may be refrigerated up to 7 days.

To see the variety of Petrifilm™ available, see our website.

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The Constant Student: The Importance of Continuing Education in the Food Industry

Books and appleBenjamin Franklin is famously quoted as saying, “An investment in knowledge pays the best interest.” In the food industry, this old adage is put to the test each time consumers open up a food product or sit down for a meal. The ability to create a safe and high-quality product demands critical thought, innovation, vigilance, and a willingness to seek out best practices. The return on this investment comes in creating a product one can be proud of and in protecting the best interests and health of one’s customers.

Yet, making sense of regulations, rules, and best practices can be somewhat daunting for the average producer. Small and medium-sized operations especially may view something as comprehensive as the Food Safety Modernization Act as an imposing and confusing path they are not sure how to navigate. So, how can one balance the want for excellence with these sometimes seemingly overwhelming demands placed on food industry interests? Simply put, the best weapon against being overwhelmed comes through education.

Nelson-Jameson is working with Cherney Microbiological Services to provide accessible educational opportunities for operations of all sizes and levels of experience. This April 21st-22nd, consider taking part in “Sanitation and Environmental Monitoring Essentials,” a course for employees involved in facility control programs where attendees will learn about food microbiology, sanitation, and environmental programs in an interactive environment. The course will be held in Green Bay, Wisconsin and an early registration discount is available through March 25th ($1195 early or $1295 after March 25th).

Contact us for additional information or to learn more about the course. We would like to know your ideas/thoughts on needed topics that you would like to see addressed in future courses and/or training opportunities, leave a reply in the comments section below or contact us at sales@nelsonjameson.com.

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